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DNA sequencing with chain-terminating inhibitors

Frederick Sanger,S Nicklen,Alan Coulson

DNA

Sanger sequencing

Nucleotide

1977

A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.

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“DNA sequencing with chain-terminating inhibitors” is a paper by Frederick Sanger S Nicklen Alan Coulson published in 1977. It has an Open Access status of “green”. You can read and download a PDF Full Text of this paper here.